Table 4.
Stability of trans-resveratrol, R3G, R3S, and IS under several conditions.
| Analytes | Stability under various conditions (mean (RSD%), % of nominal concentration) (n = 4) | ||
|---|---|---|---|
| Low QCs | Middle QCs | High QCs | |
| Short-term stability (ambient for 2 h) | |||
| Trans-resveratrol | 101.32 (8.78) | 101.62 (10.05) | 93.15 (4.83) |
| R3G | 106.18 (9.38) | 96.80 (3.46) | 97.83 (10.35) |
| R3S | 101.13 (5.94) | 92.85 (4.23) | 93.35 (8.98) |
|
| |||
| Light stability (for 2 h) | |||
| Trans-resveratrol | 108.25 (4.85) | 99.00 (10.88) | 90.70 (4.11) |
| R3G | 97.30 (6.92) | 92.43 (3.80) | 95.77 (1.79) |
| R3S | 95.75 (5.50) | 90.78 (4.37) | 99.33 (1.61) |
|
| |||
| Autosampler stability (for 12 h) | |||
| Trans-resveratrol | 91.05 (4.68) | 105.60 (7.77) | 93.20 (9.52) |
| R3G | 91.52 (4.51) | 89.34 (5.60) | 89.47 (9.19) |
| R3S | 99.02 (5.37) | 92.28 (5.53) | 94.22 (8.67) |
|
| |||
| Freeze (−80°C)-thaw (25°C) for three cycles (12 h for one circle) | |||
| Trans-resveratrol | 98.63 (13.20) | 88.83 (2.67) | 110.40 (4.13) |
| R3G | 91.33 (8.09) | 88.18 (2.48) | 87.18 (1.97) |
| R3S | 94.76 (4.79) | 93.18 (4.56) | 98.92 (6.91) |
|
| |||
| Long-term stability(−80°C for 30 days) | |||
| Trans-resveratrol | 106.44 (9.98) | 106.74 (7.64) | 95.84 (6.50) |
| R3G | 102.16 (10.95) | 97.42 (9.88) | 95.48 (5.21) |
| R3S | 101.45 (9.85) | 94.45 (5.10) | 87.23 (2.60) |
|
| |||
| Stocked solutions stability (4°C for 30 days) | |||
| Trans-resveratrol | 92.79 (1.77) | ||
| R3G | 90.37 (3.10) | ||
| R3S | 94.00 (2.89) | ||
| IS | 104.09 (8.96) | ||
R3G, trans-resveratrol-3-o-β-glucuronide; R3S, trans-resveratrol-3-sulfate salt; IS, internal standard; QCs, quality control samples.