Figure S1.

Anti-SARS-CoV-2 Ig antibody responses in plasma and saliva following BNT162b2 vaccination, related to Figure 1

Anti-SARS-CoV-2 N, RBD, and spike (S) IgG (A), IgM (B), and IgA (C) responses are shown for plasma from individuals who received BNT162b2 prime (D0, n = 59) and second dose (D21, n = 58) vaccination. Box-whisker plots of the WHO binding arbitrary unit (BAU/mL) anti-SARS-CoV-2 concentrations show the interquartile range as the box and the whisker ends as the most extreme values within 1.5 times the interquartile range below the 25% quantile and above the 75% quantile. Comparisons between groups of previously SARS-CoV-2-infected (CoV-2+) versus noninfected individuals, and female versus male were by the two-sided Wilcoxon rank sum test; comparison between age groups (<40; 40–60; >60 years) was done using pairwise Wilcoxon rank sum test with Bonferroni correction. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001.

(D) Anti-SARS-CoV-2 N, RBD, and S IgG concentrations in BAU/mL are shown for saliva from individuals who received BNT162b2 prime/boost and third dose vaccination (upper left panel). Anti-SARS-CoV-2 N, RBD, and S (upper right panel) concentrations in BAU/mL, as well as anti-SARS-CoV-1 and anti-HCoV-OC43, -HKU1, -NL63, and -229E S IgG (lower panel) concentrations in MSD arbitrary units (AU/mL), are shown for saliva collected on D42 after BNT162b2 prime vaccination (vaccinee), around D42 post-symptom onset for COVID-19 patients (CoV-2+), and before the onset of the COVID-19 pandemic for pre-pandemic healthy human controls (Pre-pan). Box-whisker plots of anti-SARS-CoV-2 IgG concentrations show the interquartile range as the box and the whisker ends as the most extreme values within 1.5 times the interquartile range below the 25% quantile and above the 75% quantile. Statistical test for significance between groups (CoV-2+; Pre-pan, vaccinee) was performed using pairwise Wilcoxon rank sum test with Bonferroni correction. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001.