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. 2021 Dec 14;25(1):103525. doi: 10.1016/j.isci.2021.103525

Figure 5.

Figure 5

The reprogramming efficiency of Nanog-GFP MEF using retrovirus vector-based L507A mutants with pro-reprogramming factors

(A) Typical morphology of Nanog-GFP colonies in the reprogramming Nanog-GFP MEF on day 25 of reprogramming in KLF4 wildtype (WT) or L507A mutant with pro-programming factors. Scale bars, 200 μm.

(B) The number of Nanog-GFP colonies in the reprogramming Nanog-GFP MEF calculated on day 25 of reprogramming in KLF4 WT or L507A mutant with pro-programming factors. Results are mean and SE, n = 4. p values calculated with the t test were shown based on the comparison between WT and L507A KLF4 conditions.

(C) The ratio of Nanog-GFP colonies to the total colonies in the reprogramming Nanog-GFP MEF was calculated on day 25 of reprogramming in KLF4 WT or L507A mutant with pro-programming factors. Results are mean and SE, n = 4. p values calculated with the t test were shown based on the comparison between WT and L507A KLF4 conditions.