Figure 2.

ScaleSF is an isometric and rapid optical clearing method

(A) The schedule for tissue clearing with ScaleSF.

(B and C) Transmission images of 1-mm-thick brain slices before (left) and after (right) treatment with ScaleSF (B) and ScaleSQ(0) (C). The grid interval is 1 mm. Scale bars, 2 mm.

(D) Changes in the size of brain slices after ScaleSF and ScaleSQ(0) treatment (n = 3, ScaleSF; n = 4, ScaleSQ(0); t = 3.261, df = 5, P = 0.0224, two-tailed unpaired Student's t test). Data are represented as means ± SDs.

(E) Transmission curves of the PBS-stored control, ScaleSF-, and ScaleSQ(0)-treated mouse brain slices (n = 3 brain hemispheres each). Data are represented as means.

(F) Three-dimensional volume rendering of the cerebral cortex of a PV-FGL mouse cleared with ScaleSF. In the PV-FGL mouse, somatodendritic membrane-targeted EGFP expression is driven by a parvalbumin promoter. Scale bar, 500 μm.

(G and H) xy images in (F) at the depths of 250 μm (G) and 750 μm (H). pia: pia mater, WM: white matter. Scale bars, 200 μm.

(I and J) Enlarged views of rectangles in (G) and (H). Scale bars, 40 μm.

See also Figures S1–S3.