Figure 1.

lncSnhg7 expression is increased in CD4⁺ T cells with activation. (A) Mice were exposed to Cd during prenatal development. Lightning bolts indicate times where parents are administered CdCl₂ (10 ppm) via drinking water. (B) Volcano plot for the comparison of gene expression between unstimulated and stimulated CD4⁺ T cells. CD4⁺CD25^- T conventional (T conv) cells were isolated from the total splenocytes of control offspring. T conv cells were cultured for 0 or 16 hours in the presence of anti-CD3/CD28 magnetic beads, unstimulated and stimulated, respectively. RNA was isolated and expression was analyzed by RNAseq. Blue background represents all genes (coding and non-coding). “+” indicates lncRNAs detected as differentially expressed in our system (FC > 2 and FDR < 0.01). (C) LncSnhg7 expression is increased in male and female activated CD4⁺ T cells. CD4⁺CD25^- T conventional (T conv) cells were isolated from control and Cd-exposed offspring and stimulated as in (B) LncSnhg7 expression was evaluated by qPCR. Statistical significance was assessed using one-tailed, paired t-test between stimulated and unstimulated samples. *p < 0.05, **p < 0.01. n=3-4 per group.