Figure 2.

GALNT7 protein expression, but not mRNA expression, is altered in CD4⁺ T cells following activation. (A) Model of GALNT1 and GALNT7 regulation by lncSnhg7. (B, C) CD4⁺CD25^- T conventional cells were isolated from total splenocytes. Cells were cultured in the presence of anti-CD3/CD28 magnetic beads for 0 and 16 hours for RNA or 0 and 72 hours for protein. GALNT1 and GALNT7 expression was analyzed by qPCR (B) and western blot (C). Statistical significance was assessed using ratio paired t-tests between stimulated and unstimulated samples with correction for multiple comparisons using the Holm-Šídák method in post-hoc analysis. *p < 0.05. n = 3-7 per group.