Figure 4.

Direct exposure to Cd does not reproduce effects of prenatal Cd exposure. Male and Female mice (8 weeks) were exposed to 21 days of CdCl₂ via drinking water. CD4⁺CD25^- T conventional cells were isolated from total splenocytes. Cells were cultured in the presence of anti-CD3/CD28 magnetic beads for 0 and 16 hours prior to RNA isolation. lncSnhg7 (A), GALNT1 (B), and GALNT7 (C) expression was analyzed by qPCR. using ratio paired t-tests between stimulated and unstimulated samples with correction for multiple comparisons using the Holm-Šídák method in post-hoc analysis. *p < 0.05. n = 3-4 per group.