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. 2022 Jan 11;12:720635. doi: 10.3389/fimmu.2021.720635

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Copyright © 2022 McCall, Varney, Rice, Dziadowicz, Hall, Blethen, Hu, Barnett and Martinez

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Activated T Cells from Cd offspring have increased proliferation as compared to control offspring, but no differences in apoptosis. (A) Splenic CD4⁺CD25^- T cells were labeled with Cell Trace Violet and stimulated with anti-CD3/CD28 magnetic beads for 72 h. Division index was calculated using FSC Express. A one-tailed, unpaired t-test was used to compare groups (n=5-8 per group); *p < 0.05. (B, C) Unlabeled T conventional cells were stimulated for 72 hours. Apoptosis was assessed by Annexin V/7AAD staining (B) and caspase 3/7 activity (C).