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Primers for qPCR.
| Target | Product Length | Primers | Tm (°C) | % Efficiency |
|---|---|---|---|---|
| mSNHG7 (NR_024068) | 149 | F: 5’-CAGAAAGAAAGCGCCTGTTG-3’ | 60 | 102.8 |
| R: 5’-GCATACCTCAGGCACGTGAT-3’ | ||||
| mGALNT1 (NM_013814) | 111 | F: 5’-CAGAAGGAAAGGTGACAGGAC-3’ | 60 | 105.5 |
| R: 5’-ATTCCAGCATCGTATGTTCCA-3’ | ||||
| mGALNT7 (NM_144731) | 129 | F: 5’-TGTTATTTGTGCCTTGTTCTCG-3’ | 60 | 104.4 |
| R: 5’-ACCAGACTTCCACGACTCTA-3’ | ||||
| mβactin (NM_007393) | 147 | F: 5’-GATTACTGCTCTGGCTCCTAG-3’ | 60 | 104.5 |
| R: 5’-GACTCATCGTACTCCTGCTTG-3’ |
