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. 2022 Jan 11;12:811616. doi: 10.3389/fimmu.2021.811616

Figure 1.

Figure 1

Preparation, characterization, and bioactivity of CMCS-20H nanoparticles. (A) SDS-PAGE and (B) WB analyses of gcIFN-20H. Lane M: protein marker. Lane 1: the fermentation supernatant of gcIFN-20H. Lane 2: the fermentation supernatant of blank vector as negative control; Lane 3: purified gcIFN-20H. (C) Transmission electron micrograph of CMCS. (D) Transmission electron micrograph of a CMCS-20H. The black arrow indicates the position of gcIFN-20H. (E) Raman spectroscopy analysis. (F) In vitro release efficiency of gcIFN-20H (plotted as a function of % cumulative release vs. time) from CMCS-20H in PBS buffer (pH = 5.2 and 7.4). (G) Antibacterial activity detection of CMCS-20H and gcIFN-20H. E. coli and S. aureus (1 × 106 CFU) were incubated with CMCS-20H, gcIFN-20H, and CMCS (20 μg/ml) for 2 h at 37°C, respectively. The equivalent volume of Tris was used as control. (H) Cytotoxicity of CMCS-20H and gcIFN-20H to CIK cells. CMCS-20H and gcIFN-20H (final concentration at 256 µg/ml) were respectively incubated with CIK cells for 24 h at 28°C. PBS was employed as control. Data are presented as means ± SD (n = 3). Different lowercase letters in each group (a and b) denote significant variations suggested by the Kruskal-Wallis statistics followed by the Dunn’s multiple comparison (p < 0.05).