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. 2022 Jan 11;12:811616. doi: 10.3389/fimmu.2021.811616

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Copyright © 2022 Huo, Wang, Xiao, Yang and Su

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Serum biochemical indexes of innate immunity assay. (A) Oral administration, challenge, and sampling schedule. Fish in each group (n = 50) were feed with CMCS-20H+fodder, gcIFN-20H+fodder, CMCS+fodder, and fodder alone (control) and challenged with 100 µl A. hydrophila (2 × 10⁵ CFU/ml) on D43. (B) The assay of total superoxide dismutase (TSOD) activity. (C) The assay of lysozyme activity. (D) The assay of complement 3 content. (E) The assay of total protein content. Serum biochemical indexes were determined by the corresponding specific commercial kits (Nanjing Jiancheng Bioengineering Institute, Nanjing, China). Data are presented as means ± SD (n = 4). Different lowercase letters in each group (a and b) denote significant variations suggested by the Kruskal-Wallis statistics followed by the Dunn’s multiple comparison (p < 0.05).