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. 2022 Jan 11;12:811616. doi: 10.3389/fimmu.2021.811616

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Copyright © 2022 Huo, Wang, Xiao, Yang and Su

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Mucus barrier investigations in grass carp intestine. (A–E) PAS staining of intestine section. The goblet cells are marked with black arrows. (a–e) Magnification of black box region to show the mucin thickness. The thickness of mucin was measured with a black line segment. (F) The number of goblet cells. Three fields (×20) were selected for goblet cell count. (G) The thickness measurement of mucin. Three fields (×70) were selected for goblet cell count. Samples were taken on D46. Different lowercase letters in each group (a and b) denote significant variations suggested by the Kruskal-Wallis statistics followed by the Dunn’s multiple comparison (p < 0.05).