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. 2022 Jan 11;12:781960. doi: 10.3389/fmicb.2021.781960

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Copyright © 2022 Mariano, Faba-Rodriguez, Bui, Zhao, Ross, Tzokov and Bergeron.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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FliF RBM2 and RBM3 stoichiometry. (A) Cryo-electron micrograph and (B) selected 2D classes of RBM1-RBM2-RBM3 (FliF_50–438). (C) A 2D class from a side view of RBM1-RBM2-RBM3 is shown, overlayed to a cartoon representation of the 33-mer FliF structure (PDBID: 6SD1). The majority of particles is side view, and matches the architecture of intact FliF. (D) Cryo-electron micrograph, and (E) selected 2D classes of RBM2 (FliF_124–229). Particles show top views and some filaments that consist of aggregation of single RBM2 disks. (F) A 2D class of a RBM2 top view is shown, overlayed to a cartoon model of the 23-mer RBM2 inner ring structure (from PDBID: 7BK0).