Table 1.

List of S. Typhimurium constructs used in this study, with their corresponding predicted MW, and that calculated by their SEC elution volume.

Domain	Construct boundaries	Monomer MW (KDa)	Oligomeric state of the purified protein	Elution volume (Ve, ml)	Apparent (by SEC) molecular weight (KDa)	Predicted molecular weight (KDa)
RBM1 + L1	50–124	8.13	Monomer	18.90	7.56	8.13
RBM2 + L2	124–229	11.21	Oligomer	9.35	390.15	257.83
RBM1 + L1 + RBM2 + L2	50–229	19.25	Monomer	17.65	11.93	19.25
RBM3	231–438	22.50	Oligomer	8.66	868.80	765.00
RBM1 + RBM2 + RBM3	50–438	41.87	Oligomer	8.27	1046.44	1422.56
Full length	1–560	63.95	-	-	-	-