Fig. 7.

miR-34a-5p targets c-MYC. A RT-qPCR tested c-MYC expression in HCT-116 cells after miR-34a-5p inhibition; B Bioinformatics website Starbase predicted the binding site of miR-34a-5p and c-MYC; C luciferase reporter gene experiment verified the targeting relationship between miR-34a-5p and c-MYC; D RT-qPCR tested DNMT3a mRNA expression in HCT-116 cells after c-MYC inhibition; E CO-IP assay tested the interaction between c-MYC and DNMT3a; F CO-IP assay tested the interaction between PTEN and DNMT3a; the data were expressed as mean ± standard deviation of three independent experiments. ^*P < 0.05 compared with the NC-inhibitor group