Table 2.
Aβ interactors isolated from tris-soluble fractions of controls, sAD and rpAD. The list of Aβ interacting partners obtained through co-IP, along with their localization, function, and distribution, is summarized (n = 4). The localization and biological functions of identified Aβ interactors were annotated using the UniProtKB database. “A” stands or sAD, “R” for rpAD, “C” for controls, “Mit” for mitochondria, “Nu” for nucleus, “Cy” for cytoplasm, “Cysk” for cytoskeleton, “Mem” for cell membrane, and “ER” for endoplasmic reticulum
| Identified proteins | UniProt ID | Localization | Functional category | Specificity |
|---|---|---|---|---|
| ATP synthase subunit beta | P06576 | Mit | Energy metabolism | C, R |
| 40S/60S ribosomal proteins | P62277 | Nu | Translation | C, R |
| Actin-related protein 2, 3 | P61160 | Nu, Cysk | Cysk organization/Axon growth | C, R |
| Adenosylhomocysteinase 2 | O43865 | ER | Translation | C |
| Adenylate kinase isoenzyme | P00568 | Cy | Energy metabolism | R |
| ADP/ATP translocase 1, 2 | P12235 | Mit | Energy metabolism | C, R |
| Band 4.1-like protein 3 | Q9Y2J2 | Mem, Cysk | Apoptosis, Cysk organization | C |
| Citrate synthase | O75390 | Mit | Glucose metabolism | C, R, A |
| Cleavage and polyadenylation specificity factor | O43809 | Nu | mRNA processing | R |
| Cysteine-rich protein 2 | P52943 | Cy | Cell division | C |
| Dihydropteridine reductase | P09417 | Mit, Cy | Redox homeostasis | R, A |
| Dihydropyrimidinase-related protein 2 | Q16555 | Cy | Axon guidance | R, C |
| Fructose-bisphosphate aldolase C | P09972 | Cy | Carbohydrate metabolism | All |
| GABA receptor-associated protein-like 2 | P60520 | Golgi | Transport | C, R |
| GTPase KRas | P01116 | Cy | Signal transduction | C, R |
| GTP-binding nuclear protein Ran | P62826 | Nu, Cy | Transport | All |
| Immunoglobulin superfamily member 8 | Q969P0 | Mem | Neurite outgrowth | C |
| LanC-like protein 1 | O43813 | Mem | Signaling | C, R |
| Microtubule-associated protein 1A | P78559 | Cysk | Cysk organization, Axonal transport | All |
| Peptidyl-prolyl cis–trans isomerase A | P62937 | Golgi | Protein refolding | A, R |
| Peroxiredoxin-2 | P32119 | Cy | Redox homeostasis | R |
| Phosphoglycerate kinase 1 | P00558 | Cy | Carbohydrate metabolism | All |
| Quinone oxidoreductase | Q08257 | Cy | mRNA processing | R |
| Serine/threonine-protein phosphatase PGAM5 | Q96HS1 | Mit | Necrosis | R |
| Synaptotagmin-1 | P21579 | Mem | Neurotransmission | C, R |
| Tubulin beta-3 chain | Q13509 | Cysk | Axon maintenance | C, R |
| Voltage-gated potassium channel subunit beta-2 | Q13303 | Mem | Neurotransmission | All |