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. 2022 Jan 11;12:817604. doi: 10.3389/fimmu.2021.817604

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Copyright © 2022 Radakovics, Battin, Leitner, Geiselhart, Paster, Stöckl, Hoffmann-Sommergruber and Steinberger

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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TLR2 stimulatory capacity of different cell wall components. (A) Activation of indicated reporter cells by the microbial components peptidoglycan (PGN), isolated from the gram-positive B. subtilis (PGN-BS) or S. aureus (PGN-SA) or the gram-negative E. coli K12 (PGN-EK), purified lipoteichoic acid from S. aureus (LTA-SA) and the synthetic ligands Diprovocim-1 (TLR2/1), Pam3CSK4 (TLR2/1) and Fsl-1 (TLR2/6) (n = 8, each experiment performed in duplicates). Exemplary histograms of eGFP expression are shown for TLR2/1/6, TLR2/1 and TLR2/6 reporter cells. (B) TLR2 surface expression on different TLR reporter cell lines was compared by staining with an AF647-coupled anti-TLR2 antibody.