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. 2022 Jan 11;12:807448. doi: 10.3389/fpls.2021.807448

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Copyright © 2022 Sharma, Sharma, Rajput, Pandey and Upadhyay.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Spot assay of recombinant Saccharomyces cerevisiae (yeast) cells under various stress conditions. (A) Shows similar growth of both TaTLP2-B (pYES2.1-TaTLP2-B) and control vector (pYES2.1/V5-His/lacZ) containing yeast cells under control conditions. (B–F) Shows the spot assay analysis of TaTLP2-B and control vector containing yeast cells in (B) cold (4°C), (C) osmotic (30% PEG), (D) heat (37°C), (E) combined heat and osmotic (37°C and 30% PEG), and (F) salt (1M NaCl) stress conditions, respectively.