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. 2022 Jan 24;8:36. doi: 10.1038/s41420-022-00818-8

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A H522 and H460 cells were treated with/without apatinib (20 μM) and propranolol (50 μM) for 48 h. The apoptosis index was detected by flow cytometry. B The percentage of apoptotic cells in H522 and H460 cells. C Western blot revealed expression level of caspase3 and cleaved-caspase3 protein. D H522 and H460 cells were treated with/without anlotinib (2.5 μM) and propranolol (50 μM) for 48 h. The apoptosis index was detected by flow cytometry. E The percentage of apoptotic cells in H522 and H460 cells. F Flow cytometry showed the proportion of cells at different stages of cell cycle. The percentages of cell cycle phases in four groups. G Flow cytometry was used to analyze ROS levels in H522 and H460 cells. (*P < 0.05; **P < 0.01; **P < 0.001; ****P < 0.0001, compared with apatinib).