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. 2022 Jan 24;5:89. doi: 10.1038/s42003-021-02998-y

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Scanning electron microscopy of a primate cone showing the outer and inner segment as well as the calyceal process around the base of the outer segment. 3D reconstructions (b, c, e g–i), reconstructed coronal slices (c and e inserts) and optical sections (d, f, last image of g–i, and j–k) of cone photoreceptors immunolabeled for the R/G cone opsin (red in a, d–g; blue in l), espin 1 (magenta e, f), Protocadherin15 (green), cone arrestin (orange in b–d), prominin 1 (red in j), acetylated alpha tubulin (blue in g), centrin 3 (purple in h), and VLGR1 (cyan in i). l normalized intensity across the cellular border. The Pcd15 staining is located in a position external to prominin 1. Numerical data in supplementary data 2. OS outer segment, CP calyceal processes, IS inner segment, NL nuclear layer, S synapse, arrowhead: pcd15 staining expression around the periciliary membrane at the base of the cilia. Scale bars represent 2 µm in (a, c, d) and 1 µm in (e–j), and 10 µm in (b).