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. 2022 Jan 24;12:1235. doi: 10.1038/s41598-022-05360-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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M-CSF secretion supports monocyte differentiation and expansion of iron recycling macrophages in the liver following burn injury. Liver cells were isolated on day seven post injury from Spi-C reporter mice that received isotype or M-CSF neutralizing antibodies. (A) Gating strategy. (B) Cell counts, n = 4–5/group. (C) Ratio of Spi-C+ monocytes/Spi-C− monocytes, n = 4–5/group. Data shown as box plots with whiskers at min and max. *p < 0.05 burn isotype versus sham isotype and ^#p < 0.05 burn anti-M-CSF versus burn isotype, ANOVA.