Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jan 24;12:1230. doi: 10.1038/s41598-022-05398-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Low glucose concentrations improved glucose uptake capability on HGnFs. (a and b) The gene expression of GLUT1 was determined using PCR in stimulated HGnFs incubated for 72 h. HGnFs were photographed by a fluorescence microscope after being incubated with fluorescently labeled secondary antibody, and the nuclei were dyed with DAPI. (c and d) The capability of glucose uptake was measured by glucose uptake assay. After 24, 72 and 120 h of incubation, HGnFs were exposed to a medium with 100 μM 2-NBDG for 2 h, and the intensity of fluorescence was measured by a microplate reader and normalized by DNA. The stained cells were photographed by a fluorescence microscope. (Scale bars: 100 μm; A significant increase compared with the control was described as **P < 0.01).