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. 2022 Jan 24;8:33. doi: 10.1038/s41420-022-00823-x

Fig. 2. miR-181a–2–3p could be carried to SH-SY5Y cells through MSC–EVs.

Fig. 2

A Abundance of miR-181a–2–3p in MSCs and MSC-derived EVs. B miR-181a–2–3p expression by RT-qPCR. C EVs observed under the TEM. D EV diameter detected using dynamic light scattering. E EV surface markers detected using Western blot. F Uptake of MSC–EV by SH-SY5Y cells detected using EV PKH67 tracing method. G Cy3-labeled miR-181a–2–3p entered SH-SY5Y cells via MSC–EV (200×). H miR-181a–2–3p expression in the SH-SY5Y cells. *p < 0.05 vs. SH-SY5Y blank, ***p < 0.001, NS insignificant difference. Cell experiments were repeated three times independently.