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. 2022 Jan 24;8:33. doi: 10.1038/s41420-022-00823-x

Fig. 9. MSC–EVs carrying miR-181a–2–3p inhibit neuronal loss and OS injury in the SN area of PD mice.

Fig. 9

A The PKH67-labeled EVs were injected intravenously into mice. Colocalization of EVs (green) with dopaminergic neurons in the SN tissues (the left panel). α-SYN expression (red) determined by immunofluorescence. DAPI (blue) located in the nucleus (scale bar = 15 μm). B miR-181a–2–3p expression in SN tissues detected using RT-qPCR. C EGR1, NOX4, p–p38, and p38 levels in SN tissues detected using Western blot analysis. D Pathological changes of SN tissues determined with H&E staining. The arrow represents the lost neurons (200 ×). E APO-induced asymmetric rotation in PD mice injected with EVs after 8 weeks. F TH expression in mouse SN tissues detected by immunohistochemical staining. G TH expression in mouse SN tissues detected using Western blot analysis. H OS marker 4-HNE in mouse SN tissues. I The dopaminergic neuron apoptosis in SN tissues detected by immunofluorescence staining. J The motor ability of mice detected by roller test. K The motor ability of mice determined by rod-climbing pole test *p < 0.05 vs. sham-operated mice, #p < 0.05 vs. saline; $p < 0.05 vs. MSC–EV–NC mimic.