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. 2022 Jan 24;12:1207. doi: 10.1038/s41598-022-05228-5

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Immunofluorescence staining for TUBB3 in neurons. (A) Representative confocal 63X and 180X images (above) and respective 63X and 180X skeletonized images (below) of TUBB3 positive neurons at T4 (28 weeks) (B) The results are expressed as mean ± S.D. NC NW: Normal Chow Normal Water; WD SW: Western Diet Sugar Water. T0: 0 weeks; T2: 8 weeks; T4: 28 weeks. TUBB3: anti-β III Tubulin. *: p < 0.05, **: p < 0.01, ***: p < 0.001 vs NC NW T4; °: p < 0.05, °°: p < 0.01 vs WD SW T0; #: p < 0.05 vs WD SW T2.