TABLE 1.
List of the confirmed N-glycans derived from VRC01 mAb analyzed in the study. Also included are the observed retention times, normalized abundance (% amount), expected and observed GU values, expected and observed masses, the predominant charge state, and the response units. The highlighted glycan species are those that are shortlisted to be monitored using the screening workflow.
| SL. No | Component name | Observed RT (min) | Glycan units | Observed mass (Da) | % Amount (%) | Expected glycan units | Expected mass (Da) | Charge | Response |
|---|---|---|---|---|---|---|---|---|---|
| 1 | M3 | 9.04 | 4.3583 | 1,221.507 | 0.43 | 4.3633 | 1,221.502 | 2 | 31886133 |
| 2 | F(6)M3 | 10.27 | 4.752 | 1,367.564 | 0.81 | 4.7617 | 1,367.56 | 2 | 60887988 |
| 3 | A1 | 10.9 | 4.9581 | 1,424.587 | 0.33 | 4.9699 | 1,424.582 | 2 | 24741016 |
| 4 | M4 | 11.72 | 5.2148 | 1,383.558 | 0.46 | 5.3077 | 1,383.555 | 2 | 34666288 |
| 5 | F(6)A1 | 12.03 | 5.3126 | 1,570.643 | 1 | 5.2906 | 1,570.64 | 2 | 75026269 |
| 6 | A2 | 12.64 | 5.5003 | 1,627.665 | 0.42 | 5.4619 | 1,627.661 | 2 | 31299243 |
| 7 | M4A1 | 13.5 | 5.7619 | 1,586.637 | 0.32 | 5.7402 | 1,586.635 | 2 | 24184490 |
| 8 | F(6)A2 | 13.68 | 5.8169 | 1,773.727 | 4.98 | 5.7879 | 1,773.719 | 2 | 3.74E+08 |
| 9 | F(6)A1[3]G(4)1 | 14.54 | 6.0808 | 1,732.698 | 1.24 | 6.095 | 1,732.692 | 2 | 93050430 |
| 10 | M5 | 14.92 | 6.1996 | 1,545.611 | 5.75 | 6.1692 | 1,545.608 | 2 | 4.31E+08 |
| 11 | A2[3]G(4)1 | 15.47 | 6.3716 | 1,789.718 | 0.36 | 6.29 | 1,789.714 | 2 | 27222853 |
| 12 | F(6)A2[6]G(4)1 | 16.05 | 6.559 | 1,935.777 | 2.87 | 6.5337 | 1,935.772 | 2 | 2.15E+08 |
| 13 | F(6)A2[3]G(4)1 | 16.46 | 6.6921 | 1,935.779 | 2.3 | 6.6633 | 1,935.772 | 2 | 1.72E+08 |
| 14 | F(6)A2[6]BG(4)1 | 16.83 | 6.8134 | 2,138.856 | 0.66 | 6.8 | 2,138.851 | 2 | 49258887 |
| 15 | M6 | 17.54 | 7.0486 | 1,707.662 | 0.61 | 7.1138 | 1,707.661 | 2 | 45834299 |
| 16 | A2G(4)2 | 17.81 | 7.1397 | 1,951.772 | 4.05 | 7.0955 | 1,951.767 | 2 | 3.04E+08 |
| 17 | M6 D1 | 18.07 | 7.2303 | 854.8377 | 0.21 | 7.14 | 821.7959 | 2 | 15499336 |
| 18 | A2BG(4)2 | 18.52 | 7.3836 | 2,154.865 | 0.78 | 7.35 | 2,154.846 | 3 | 58458127 |
| 19 | F(6)A2G(4)2 | 18.69 | 7.4413 | 2,097.832 | 8.91 | 7.4266 | 2,097.825 | 2 | 6.68E+08 |
| 20 | M5A1G(4)1 | 19.03 | 7.5596 | 1,910.739 | 0.69 | 7.431 | 1,910.74 | 2 | 51800792 |
| 21 | F(6)A2BG(4)2 | 19.21 | 7.6232 | 2,300.913 | 0.31 | 7.605 | 2,300.904 | 3 | 23189435 |
| 22 | F(6)A3G(4)2 | 19.69 | 7.7936 | 2,300.912 | 1.15 | 7.7585 | 2,300.904 | 3 | 86301406 |
| 23 | F(6)A2[6]G(4)1S(6)1 | 19.9 | 7.8696 | 2,226.876 | 3.7 | 7.84 | 2,226.867 | 2 | 2.77E+08 |
| 24 | M7 D1 | 20.3 | 8.0131 | 1,869.719 | 0.65 | 8.0285 | 1,869.714 | 2 | 48971172 |
| 25 | A2G(4)2S(3)1 | 20.47 | 8.0732 | 748.6280 | 1.29 | 8.06 | 729.282 | 2 | 96888102 |
| 26 | F(6)A2G(4)2S(3)1 | 20.68 | 8.1502 | 2,388.927 | 9.36 | 8.124 | 2,388.92 | 2 | 7.01E+08 |
| 27 | F(6)A2[3]G(4)1Sg(6)1 | 21.06 | 8.2939 | 2,242.868 | 0.65 | 8.3057 | 2,242.862 | 2 | 48619741 |
| 28 | F(6)A2G(4)2S(6)1 | 21.65 | 8.5146 | 1,195.4673 | 0.14 | 8.55 | 1,394.5757 | 2 | 10806796 |
| 29 | A2G(4)2S(3,3)2 | 21.83 | 8.5856 | 2,533.966 | 3.97 | 8.57 | 2,533.958 | 3 | 2.97E+08 |
| 30 | A2[3]G(4)1S(6)1 | 22.11 | 8.6935 | 694.6104 | 0.1 | 8.69 | 586.5778 | 2 | 7558824 |
| 31 | F(6)A2BG(4)2S(6)1 | 22.3 | 8.7666 | 2,592.009 | 0.67 | 8.82 | 2,591.999 | 3 | 50024309 |
| 32 | F(6)A2G(4)2S(3,3)2 | 22.47 | 8.8339 | 2,680.025 | 11.92 | 8.85 | 2,680.016 | 3 | 8.94E+08 |
| 33 | A2G(4)2S(3,6)2 | 22.92 | 9.0107 | 2,533.965 | 0.33 | 9 | 2,533.958 | 3 | 24788235 |
| 34 | A3G(4)3S(3)1 | 23.33 | 9.1737 | 2,608.012 | 1.57 | 9.23 | 2,607.994 | 3 | 1.18E+08 |
| 35 | A2G(4)2S(6,6)2 | 23.8 | 9.3668 | 845.6598 | 1.48 | 9.415 | 869.3230 | 2 | 1.11E+08 |
| 36 | F(6)A3G(4)3S(3)1 | 24.02 | 9.4614 | 2,754.06 | 0.68 | 9.538 | 2,754.052 | 2 | 50906409 |
| 37 | F(6)A2G(4)2S(6,6)2 | 24.21 | 9.5405 | 2,680.015 | 0.47 | 9.69 | 2,680.016 | 2 | 35023310 |
| 38 | M9 | 24.47 | 9.6488 | 2,193.825 | 1.32 | 9.6667 | 2,193.819 | 2 | 98775879 |
| 39 | F(6)A4G(4)4 | 24.92 | 9.8416 | 2,828.098 | 2.12 | 9.915 | 2,828.089 | 3 | 1.59E+08 |
| 40 | F(6)A3Lac3 | 25.05 | 9.8994 | 1,537.1048 | 0.68 | 9.91 | 1,529.1198 | 2 | 50960194 |
| 41 | F(6)A3G(4)3Lac1 | 25.33 | 10.019 | 2,828.1 | 1.72 | 10.015 | 2,828.089 | 3 | 1.29E+08 |
| 42 | A3S(6)1G(4,4,3)3S(3,6)2 | 26.04 | 10.3369 | 3,190.18 | 1.1 | 10.31 | 3,190.185 | 3 | 82361071 |
| 43 | M10 a3D1,a3D3,a2D4(2) | 26.42 | 10.5103 | 2,355.882 | 2 | 10.53 | 2,355.872 | 2 | 1.5E+08 |
| 44 | F(6)A3G(4)3S(3,3,3)3 | 26.62 | 10.6002 | 3,336.262 | 2.97 | 10.6267 | 3,336.243 | 3 | 2.23E+08 |
| 45 | A3G(4)3S(3,3,6)3 | 26.99 | 10.774 | 2,879.0101 | 0.51 | 10.72 | 2,862.6845 | 3 | 38209650 |
| 46 | F(6)A3G(4)2Ga(3)2Sg(6)1 | 27.29 | 10.9168 | 978.3740 | 1.99 | 10.94 | 940.687 | 2 | 1.49E+08 |
| 47 | F(6)A4G(4)4Lac1 | 27.53 | 11.0304 | 3,193.235 | 0.14 | 11.01 | 3,193.221 | 3 | 10417180 |
| 48 | F(6)A3G(4)3Lac2 | 27.79 | 11.1559 | 1,065.41 | 1.52 | 11.16 | 1,059.7256 | 2 | 1.14E+08 |
| 49 | F(6)A4G(4)4S(3,3,3)3 | 28.52 | 11.5199 | 3,701.393 | 2.02 | 11.52 | 3,701.375 | 3 | 1.51E+08 |
| 50 | F(6)A3G(4)3Lac1S(3,3,3)3 | 28.81 | 11.666 | 3,701.389 | 1.39 | 11.71 | 3,701.375 | 3 | 1.04E+08 |
| 51 | F(6)A4G(4)4S(3,3,3,3)4 | 29.73 | 12.148 | 3,992.491 | 3.7 | 12.17 | 3,992.471 | 3 | 2.77E+08 |
| 52 | F(6)A3G(4)3Lac2S(3,3,3)3 | 30.47 | 12.5441 | 4,066.515 | 0.66 | 12.52 | 4,066.508 | 3 | 49654386 |
| 53 | F(6)A4G(4)4Lac1S(3,3,3)3 | 30.73 | 12.6875 | 4,066.512 | 0.54 | 12.7 | 4,066.508 | 3 | 40618466 |
Abbreviations: F, fucose; G, galactose; A1, monoantennary; A2, biantennary; Sg, N-glycolylneuraminic acid; Ga, a1,3-linked galactose.