FIGURE 1.

Dysregulated circRNAs in chemoresistant gastric cancer (GC). (A) Schematic illustration of screening the autophagy‐related ceRNA regulatory network in regulating 5‐fluorouracil (5‐FU) resistance. (B) Volcano plot for the mRNA matrix generated on the basis of genomics of drug sensitivity in cancer (GDSC) statistics. (C) Left panel: Pearson correlation between genes and 5‐FU sensitivity according to the GDSC database. Right panel: PRKAA2 was negatively correlated with the IC50 values for 5‐FU. (D) The Kaplan‐Meier (KM) plotter data of gastric cancer patients receiving 5‐FU‐based chemotherapy. (E) Kaplan–Meier analysis of the correlation between PRKAA2 expression and overall survival. (F) Expression of PRKAA2 in 5‐FU‐resistant and 5‐FU‐sensitive GC tissues by qRT‐PCR. (G) CCK8 assay of the effect of silencing PRKAA2 on the drug sensitivity of AGS‐5FU cells. (H) Expression of five‐candidate circRNAs in 5‐FU‐resistant and 5‐FU‐sensitive GC tissues by qRT‐PCR. (I) Kaplan–Meier analysis of the correlation between circ0027497 expression and overall survival. (J) CCK8 assay of the effect of silencing circ0027497 on the drug sensitivity of AGS‐5FU cells. (K) Pearson correlation analysis between the expression of circ0027497 and PRKAA2