Skip to main content
. 2022 Jan 24;12(1):e708. doi: 10.1002/ctm2.708

FIGURE 6.

FIGURE 6

Circular CPM (CircRNA) regulates gastric cancer (GC) chemoresistance through PRKAA2‐mediated autophagy. (A and C) apoptotic assays of AGS‐5FU cells transfected with si‐circ or co‐transfected with PRKAA2 overexpression vector upon 5‐FU (25 μM 48 h) exposure. (B and E) Apoptotic assays of AGS transfected with circCPM overexpression vector or co‐transfected with sh‐PRKAA2 upon 5‐FU (5 μM 48 h) and/or chloroquine (CQ) (20 μM 24 h) exposure. (D and G) Western blot analysis of caspase3, c‐caspase 3, LC3 and p62 in AGS‐5FU transfected with si‐circ or co‐transfected with PRKAA2 overexpression vector upon 5‐FU (25 μM 48 h) exposure. (F and H) Western blot analysis of caspase3, c‐caspase 3, LC3 and p62 in AGS transfected with circCPM overexpression vector or co‐transfected with sh‐PRKAA2 upon 5‐FU (5 μM 48 h) and/or CQ (20 μM 24 h) exposure. (I‐N) Immunofluorescence analysis (scale bar 20 μm) and transmission electron microscopy (TEM) images (scale bar = 2 μm or 0.5 μm) of AGS‐5FU and AGS. (I and L) Quantification data of autolysosome and autophagosome and autophagic vacuoles (AV) counts in AGS‐5FU transfected with si‐circ or co‐transfected with PRKAA2 overexpression vector upon 5‐FU (25 μM 48 h) exposure. (J, K and M) Quantification data of autolysosome and autophagosome and AV counts in AGS transfected with circCPM overexpression vector or co‐transfected with sh‐PRKAA2 upon 5‐FU (5 μM 48 h) and/or CQ (20 μM 24 h) exposure. (Graph represents mean ± SD; *p < .05, **p < .01 and ***p < .001)