Figure 5.

Progression of cdc4Δ mutant infection in lungs of infected mice. Lungs of the mice infected by cdc4Δ mutant and the wild type were isolated at 1, 3, 5, 7, 9,11, 13, and 15 days after infection. The number of CFU was measured in lung homogenates (A). The data shown are the mean ± SD for values of five mice. ns, not significant. ***P < 0.001. ****P < 0.0001 (determined by Mann-Whitney test). (B) Methenamine silver-stained slides were also prepared from the cross-section of the lung and observed by light microscopy. The yeast cells are indicated by arrows. Bar, 20 µm.