Figure 6.

Cdc4 plays an important role in the proliferation inside the macrophage and the survival in the host complement system. (A) The proliferation of Cryptococcus inside the macrophage was performed using the J774 murine macrophage cells. After incubation of indicated time, the lysates of the macrophage cultures were spread on YPD plates, and the CFU counts were used to measure the intracellular proliferation and macrophage killing. *P < 0.05; **P < 0.01 (determined by Mann-Whitney test). As a control, the growth of the wild type and the cdc4Δ mutant was also measured in DMEM without macrophages (B). (C) Overnight cultures of the same strains as in (A) were mixed with mouse serum and incubated at 37 °C for indicated times. One hundred microliters of the dilute (10³ dilution) was spread on YPD plates, and the CFU counts were used to measure the Cryptococcus cell viability. ns, not significant; ***P < 0.001 (determined by Mann-Whitney test).