Figure 3.

No correlation between antibody levels cross-reactive SARS-CoV-2 S1 subunit or RBD and neutralization activity. (A) Infection with SARS-CoV-2 pseudotype viruses. (B) Confirmation of RBD specific infection with anti-SARS-CoV-2 RBD (black dots) or anti-HA (red dots) antibodies. (C) Dot plot showing the four groups divided using median split (3.63 = log₁₀(AU/ml) for RBD and 2.87 = log₁₀(AU/ml) for S1 subunit). (D) Correlation analysis between anti-SARS-CoV-2 S1 subunit and anti-RBD IgG antibody levels and neutralization. (E) Box plot representing the neutralization activity level. Gray dotted lines denote 25% neutralization (upper) and -25% neutralization (lower). Pearson’s correlation coefficient (r) and its associated P-value (P). NS, not significant (unpaired Student’s t-test).