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. 2022 Jan 11;12:783790. doi: 10.3389/fgene.2021.783790

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Copyright © 2022 Wang, Zhang, Tang, Zheng, Li, Yang and Xiang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression and Location Analysis of CFAP251 Protein in Sperm. (A) Immunoblotting analysis of protein in sperm lysates from a normal control and from men harboring the CFAP251 mutation. GAPDH was used as a loading control. (B) The spermatozoa were stained with anti-CFAP251 (red) and anti-α tubulin (green) antibodies. Tubulin antibody staining indicates flagella, and DAPI staining indicates the nucleus of spermatozoa. Compared with the normal control, CFAP251 protein is absent from the sperm flagella of patients.