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. 2021 Nov 9;205(2):208–218. doi: 10.1164/rccm.202008-3303OC

Figure 1.

Figure 1.

Immunofluorescence staining and relative protein abundance of the alveolar cell marker proteins in the tandem mass tag (TMT) proteomics dataset proteins during postnatal development. (A) Confocal immunofluorescence staining of AGER (green), PECAM1 (red), NKX2.1 (white), and DAPI (blue) for a 4-year-old donor. (B) Confocal immunofluorescence staining of SFTPC (green), ABCA3 (red), ACTA2 (white), and DAPI (blue) for a 3-year-old donor. (C–F) Relative abundance of the alveolar cell marker proteins measured by proteomics: AGER_HUMAN (C), ABCA3_HUMAN (D), ACTA_HUMAN (E), and PSPC_HUMAN (F). Likelihood ratio tests were conducted to identify the proteins with evidence of significant linear increases with age (*P < 0.05 and **P <0.01). (G) Western blot performed for RAGE. (H) Quantitative assessment made on the Western blots normalized by the abundance of GAPDH (*Student’s t test P = 0.0131; bars represent the mean values, and error bars are SE). Scale bars, 100 μm.