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. 2021 Nov 9;205(2):208–218. doi: 10.1164/rccm.202008-3303OC

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Figure 4. — Proteasomal proteins are downregulated during postnatal lung development. (A) Heat map depicting the abundance of the standard proteasome subunits and of the 19S regulatory particle. (B) Heat map depicting the abundance of the immune-proteasome subunits and of the PA28-ɑβ regulatory particle. (C) Western blot of the PSB5/β5 subunit of the proteasome (top panel) and the total protein abundance (bottom panel). (D) PSB5 abundance normalized by the total loaded protein intensity from Western blotting. (E) Western blot of the PSB8/iβ5 subunit of the proteasome (top panel) and the total protein abundance (bottom panel). (F) PSB8 abundance normalized by the total loaded protein intensity from Western blotting. (G–I) Measured proteasome caspase-like (G), tryptic-like (H), and chymotrypsin-like (I) activities expressed in relative light units (RLU). *P < 0.05.