FIGURE 4.

Maximum likelihood concatenated marker gene tree showing the phylogenetic placement of GAL08 within the domain Bacteria. The tree includes eleven GAL08 SAGs, five GAL08 MAGs, 68 Acidobacteria genomes, 214 genomes belonging to 6 other phyla, and 6 genomes from the Parcubacteria, serving as the outgroup. The 214 genomes were selected from a reduced set of bacterial isolate genomes available in the Integrated Microbial Genomes (IMG) database (Chen et al., 2021). The total set of (61,619) available genomes was first reduced by clustering the RNA polymerase beta-subunit gene at 65%; then, the reduced set was further minimized by screening for genomes belonging to phyla to which the GAL08 16S rRNA gene sequence shows the closest 16S rRNA gene identity (Thermotogae, Aquificae, Thermodesulfobacteria, Firmicutes, Actinobacteria, and Bacteroidetes, at 82–84%); the final set served as a reference database for downstream tree inference.