Figure 4.

Inhibitory activity of OM on TLR3 and TLR7/8 signaling pathways in THP‐1 cell‐derived macrophages. a) A scheme demonstrating the inhibitory effect of the PPIs on endosomal TLR activation. OM significantly inhibited NF‐κB/AP‐1 (left) and IRF (right) activation stimulated by b) PolyI/C (50 µg mL⁻¹) on TLR3 and c) R848 (10 µg mL⁻¹) on TLR7/8 signaling. d) OM could not inhibit NF‐κB/AP‐1 activation induced by Pam3CSK4 (10 ng mL⁻¹, TLR2 signaling). Inhibition of IL‐6 and MCP‐1 production by OM upon e) PolyI/C and f) R848 stimulation. OM = 50 µg mL⁻¹, N = 3, ns: not significant, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.