TABLE 1.
PCR and MSE values obtained with different primer pairsa
| Strain | Band no. (PCR product size, MES [bp])
|
|||||
|---|---|---|---|---|---|---|
| sp2-sp17 (55°C) | sp5-sp13 (55°C) | sp7-sp17 (60°C) | sp5–IS-left (60°C) | sp1–IS-left (62°C) | sp2–IS-left (60°C) | |
| B | bd1 (250, 280) | ND | bd11 (1,110, 630) | ND | ND | |
| A | NA | bd5 (300, 210) | bd8 (400, 350) | bd12 (1,200, 980) | ND | ND |
| E | NA | bd6 (640, 560) | bd9 (680, 630) | ND | ND | ND |
| D | ND | ND | ND | bd13 (550, 350) | ND | ND |
| BCG | NA | bd7 (640, 560) | bd10 (700, 630) | bd14 (1,350, 1,260) | ND | ND |
| C | ND | ND | ND | ND | bd15 (400, 210) | bd16 (300, 140) |
a
PCR annealing temperatures are in parentheses. Values are given in base pairs. bd, band (in boldface are those selected for cloning and sequencing). NA, no amplification. ND, not done.