Figure 7.

SARS‐CoV‐2 N protein promotes the expression of p21 and cell cycle arrest in vitro. A,B) Western blotting and real‐time PCR for expression of p21 in response to overexpressing SARS‐CoV‐2 N protein and in response to H/R injury. C,D) Western blotting and real‐time PCR for expression of p21 in response to overexpressing SARS‐CoV‐2 N protein and addition of TGF‐β (5 ng mL⁻¹). E) Cell cycle progression in response to overexpressing SARS‐CoV‐2 N protein and addition of TGF‐β (5 ng mL⁻¹) by flow‐cytometry analysis. Data was expressed as means ± SEM for at least three independent experiments. Data was expressed as means ± SEM for at least three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 versus Flag‐Vector; #p < 0.05, ##p < 0.01, ###p < 0.001 versus Flag‐Vector with TGF‐β1 or H/R.