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. 2021 Nov 24;9(3):2103608. doi: 10.1002/advs.202103608

Figure 7.

Figure 7

The pro‐inflammatory response associated with actin cytoskeleton assembly of macrophages is alleviated on generated fibrin network with thinner fibers. A) GSEA reveals the global difference in 2, 8, 14, and 20 nm versus Blank of cytokine activity of GO molecular function. |NES| > 1, NOM p‐val < 0.05 and FDR q‐val < 0.25 are considered significant. B) Pearson correlation analysis of downregulated actin filament assembly genes and downregulated cytokine activity related genes. C) RT‐qPCR results of pro‐inflammatory cytokines and chemokines related gene expressions in macrophages cultured on fibrin network. D) Cytokine profiles of cell cultured supernatant of macrophages cultured on fibrin network using Inflammation Antibody Array. E) TNF‐α and IL6 concentration of cell cultured supernatant of macrophages cultured on fibrin network using ELISA. F,G) Representative immunofluorescence images and semi‐quantitative statistical analysis of CCR7 (classic M1‐type macrophage marker) in macrophages cultured on fibrin network. Data are presented as means ± s.d.; n = 3; *p < 0.05, **p < 0.01, ***p < 0.001 by one‐way ANOVA with Tukey's post hoc test.