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Stmp1 localizes to the bacterial outer membrane. (A) Stmp1 localization within the bacterial cell was determined by fractionation of Stmp1-Flag-expressing C. burnetii. Controls for the subcellular fractions were elongation factor Ts (EF-Ts) and DotB (cytoplasm), IcmD (inner membrane), and DotH/IcmK (outer membrane). Immunoblots blots are representative of three independent experiments. (B) Schematic representation of Stmp1 in C. burnetii.
