FIG 8.

PXR abrogates tilivalline-induced enterocyte tubulin acetylation. (A) Immunoblot of acetylated tubulin, total tubulin, and β-actin in T84 cells stably transfected with empty vector pCDNA3 (EV) or human PXR (hPXR). Cells were treated with tilivalline (100 μM and 200 μM) or paclitaxel (PTX) (10 μM), an established tubulin-acetylating/polymerizing agent whose toxic effects are modulated by PXR; cell lysates were transferred to nitrocellulose membranes and probed with antibodies specific for anti-acetylated tubulin, total tubulin, and β-actin. (B) Values of band intensities in EV and T-hPXR T84 cells with or without various treatments. The data represent the mean values ± standard errors of the means (n = 3). Statistical analysis was done by one-way ANOVA with multiple comparisons. *, P ≤ 0.05; ***, P ≤ 0.001.