FIG 5.
Genomic analysis of the acquisition of the AbaR1 island. (A) Transformation frequencies of the acquisition of tetracycline resistance carried by the AbaR1 island of the AYE strain, in a mixed-culture setup between the AYE strain impaired for natural transformation (comEC::aacC4) and the M2 Rifr strain and by supplying M2 Rifr with genomic DNA extracted from AYE. (B) Graphical representation of the chromosome of the AYE strain (thin black line) and location of the acquired DNA fragments (light gray thick lines) by transformants of the A. nosocomialis strain M2 during mixed culture with AYE. Bottom diagram, close-up view of the acquired fragments and the AbaR1 island. The genomes of tetracycline-resistant recombinants of M2 were sequenced. Variant calling and identification of converted markers (SNPs of AYE acquired by M2) were used to delineate the acquired regions (see Materials and Methods).