FIG 6.

GNP deletion strains have impaired survival and ability to damage J774 macrophages. C. albicans cultures grown overnight were confronted with J774 macrophages for 24 h. (A) Lactate dehydrogenase (LDH) release from macrophages was measured. The damage capacity of different C. albicans strains was calculated relative to the high control (complete macrophage lysis through treatment with 5% Triton for 10 min). Significance was tested via one-way ANOVA against the wild type (*, P < 0.05; **, P < 0.01; ****, P < 0.0001). The experiment was performed in technical triplicates and seven biological replicates. (B) C. albicans colonies were counted after 24 h of coincubation with macrophages as the readout for fungal survival. Survival was calculated as a ratio relative to the wild type. Significance was tested via one-way ANOVA. The experiment was performed in four biological replicates.