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Gnp2 and, to a lesser degree, Gnp1 are important for fungal resistance against oxidative stress. Serial dilutions (OD of 1, 10−1, 10−2, 10−3, 10−4, and 10−6) of C. albicans cultures grown overnight were spotted onto plates with the indicated medium in either the presence or absence of the ROS stress inducer t-BOOH. The plates were incubated for 2 days at 37°C. All assays were performed in biological triplicates. Representative pictures are shown.
