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. 2021 Dec 28;2(1):100137. doi: 10.1016/j.crmeth.2021.100137
REAGENT or RESOURCE SOURCE IDENTIFIER
Bacterial and virus strains

B. vulgatus cell culture Lab stock Bacteroides vulgatus ATCC 8482
B. subtilis cell culture Lab stock Bacillus subtilis str. 168 BS168

Biological samples

Human stool sample Korpela et al., 2018 Stool sample from baby collected in Luxembourg. https://www.ebi.ac.uk/ena/browser/view/SAMEA104445596

Deposited data

Data and scripts (CheckM, Binning, Contig KEGG-KO table, Chip designs, PADS data plotting via GitHub) This paper, deposited in the Open Science Framework https://doi.org/10.17605/OSF.IO/6TMZK
FIJI plugin code and imaging data This paper, deposited in Zenodo https://doi.org/10.5281/zenodo.5748533
Genomic data This paper, deposited in ENA Project accession number PRJEB48713; sample 1 https://www.ebi.ac.uk/ena/browser/view/ERS8538067 and sample 2 https://www.ebi.ac.uk/ena/browser/view/ERS8538068

Oligonucleotides

Forward primer with 5’ Mod: BtnTg for Bacteroides vulgatus and dorei Sigma-Aldrich CAAGCTGAGAAAAGCAGCCAAA
Probe sequence with Iowa Black FQ and intermediate ZEN quencher and 5’ 6-FAM fluorophore for Bacteroides vulgatus and dorei IDT AGTGGCAGTAGCCGGAGGGGTA
TCAGCCA
Reverse primer with 5’ Mod: BtnTg for Bacteroides vulgatus and dorei Sigma-Aldrich GAATGAGTTACGAAGCCCGTTG
Forward primer with 5’ Mod: BtnTg for Bacillus subtilis Sigma-Aldrich TCGTGCTGAGACAGTTGCTT
Probe sequence with Iowa Black FQ and intermediate ZEN quencher and 5’ 6-FAM fluorophore for Bacillus subtilis IDT TTGCGGGCGGCGGTATGGCAGGAGCT
Reverse primer with 5’ Mod: BtnTg for Bacillus subtilis Sigma-Aldrich TCTTTCCCTTCAAGGCGGAC