Table of cell encapsulation mix for PCR
| Aqueous reagent | Volume | Concentration/comment |
|---|---|---|
| Cell suspension aliquot | e.g. 50 μL | Volume adjusted according to cell suspension aliquot density to encapsulate on average one cell in every fourth or fifth droplet (ca. 30 pl/droplet) |
| 10 μM primer mix | 55 μL | 0.69 μM final concentration of each primer (forward and reverse, aliquots mixed in advance at 10 μM of each primer) |
| 100 μM TaqMan probe | 20 μL | 2.5 μM final concentration |
| Taq hotstart polymerase | 2 uL | To improve efficiency of late PCR cycles |
| 100 μM mix of each dNTP | 6 μL | 0.75 μM final concentration |
| Master mix | 440 μL | |
| Nuclease-free Water | Until 800 μL |