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. 2021 Dec 28;2(1):100137. doi: 10.1016/j.crmeth.2021.100137

Table of droplet PCR thermocycle settings

Time Temperature Conditions
95°C 15 min Hold for cell lysis (Rosa et al., 2010), from here on sample biosafety level is reduced to L1.
95°C 3 sec 45×
60°C∗ 16 sec
72°C 2 min Hold
37°C infinite

∗Each program needs to be adjusted to probes/primers annealing temperature.