Skip to main content
. Author manuscript; available in PMC: 2022 Jan 25.
Published in final edited form as: Cell Rep. 2021 Dec 28;37(13):110175. doi: 10.1016/j.celrep.2021.110175

Figure 1. Loss of LGP2 increases antiviral and TNFα responses.

Figure 1.

(A and B) 2fTGH cell lines with (WT) and without (KO) LGP2 expression were infected with 5 PFU/cell SeV for 10 h prior to RNA isolation and qRT-PCR to measure mRNA levels of: (A) IFN-β and (B) ISG15. Dot plots represent technical replicates from one of three independent experiments ± standard deviation. *p < 0.05, **p < 0.005, ***p < 0.0005 by two-tailed Student’s t test.

(C) 2fTGH cell lines with (WT) and without (KO) LGP2 expression were stimulated with TNFα and lysed at the time of stimulation (0), and after 30 min, 1 h, 2 h, 4 h, and 6 h. Lysates were subjected to immunoblot with antiserum for IκBα, p-IκBα (Ser32), and GAPDH.

(D–G) 2fTGH cell lines with (WT) and without (KO) LGP2 expression were stimulated for up to 6 h with TNFα prior to RNA isolation and qRT-PCR to measure mRNA levels of (D) CCL2, (E) CCL5, (F) IL-1β, and (G) LGP2 (exon 12/13). Dot plots represent technical replicates from one of three independent experiments ± standard deviation. *p < 0.05, **p < 0.005, ***p < 0.0005 by two-tailed Student’s t test. Additional qPCR experiments and bar graphs are shown in Figures S1 and S2.