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. Author manuscript; available in PMC: 2022 May 21.
Published in final edited form as: J Vis Exp. 2021 May 21;(171):10.3791/62075. doi: 10.3791/62075

Figure 4: Purification of VJM2 Pool (post Ni-NTA) using IEX via MCPA system.

Figure 4:

A. Absorbance readings of the collected elutions from the ion-exchange (IEX) were measured using a NanoDrop. The readings are plotted against the corresponding NaCl concentration of the elution. B. SDS-PAGE analysis of the IEX salt elutions presented in A. The molecular weight marker is shown on the left of the gel.