Figure 6.
Continuous recording of DHFRtag conformational changes during the catalyzed reactions. NADPH (1.0 mM) and dihydrofolate (DHF) (1.9 μM) were added to the cis and trans chambers, respectively. The pink asterisks are indicating the occurrence of the product, tetrahydrofolate. Current traces were collected in 250 mM KCl, 15 mM Tris–HCl pH 7.8 at room temperature (25 °C), by applying a Bessel low-pass filter with a 2 kHz cutoff and sampled at 10 kHz. The trace was filtered digitally with a Gaussian low-pass filter with a 100 Hz cutoff.